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. 2016 Jul 22;12(3):2163–2168. doi: 10.3892/ol.2016.4904

Figure 2.

Figure 2.

(A) Bioinformatic analysis revealed that the targeting association between miR-429 and DLC-1 is evolutionarily conserved. (B) The seed sequences for miR-429 at the wild type or mutant type 3′UTR of DLC-1 are shown. (C) Luciferase reporter assay was performed to clarify whether DLC-1 was a target gene of miR-429. The luciferase activity was reduced only in non-small cell lung cancer H1229 cells co-transfected with miR-429 mimic and wild type DLC-1 3′UTR, but unchanged in other groups. **P<0.01 vs. control. (D) Reverse transcription-quantitative polymerase chain reaction and (E) western blotting were used to determine the mRNA and protein expression levels of DLC-1 in H1229 cells transfected with miR-429 mimic, miR-429 inhibitor or scramble miR mimic as a NC. Glyceraldehyde-3-phosphate dehydrogenase was used as an internal reference. Non-transfected H1229 cells were used as a control. **P<0.01 vs. control. miR, microRNA; DLC-1, deleted in liver cancer 1; UTR, untranslated region; NC, negative control.