miR-103 levels are correlated with the metastatic capacity in CRC cell lines and CRC tissues. (A) The expression of miR-103 in the 7 colonic FHC, NCM460, SW480, HT-29, HCT-116, SW620 and LoVo cell lines was detected by RT-qPCR. (B) Expression of miR-103 in 38 CRC tissues and matched normal tissues was detected by RT-qPCR. (C) qPCR data of miR-103 levels in primary CRC lymph nodes (metastasis positive or metastasis free). (D) Overall survival curves for the two groups defined by low and high expression of miR-103 in patients with CRC. Expression of miR-103 was normalized against U6. All assays were performed in duplicate. *P<0.05, **P<0.01, ***P<0.001 vs. control. CRC, colorectal cancer, RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR-103, microRNA-103.