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. 2016 Feb 18;95(7):e2713. doi: 10.1097/MD.0000000000002713

FIGURE 1.

FIGURE 1

A drug-inducible system to efficiently delete miR-17-92 cluster in adult mouse testes. A, Targeting scheme for the miR-17-92 cluster conditional deletion. B–E, X-Gal staining showing the high efficiency of Cre-mediated recombination in testis of adult R26R/hUb-Cre-ERT2 mice. Eight week old R26R/hUb-Cre-ERT2 mice were treated by intraperitoneal injection of 20 mg/mL tamoxifen in corn oil (0.2 mg/g body weight, once per day for 5 days) to activate the Lac Z expression. F, PCR-based genotyping was performed on genomic DNA from the testes of miR-17-92-deficient mice. UT: untreated with tamoxifen; D1, D7, D14, D21 and D28 represent the 1st, 7th, 14th, 21st, and 28th days after the last tamoxifen injection in miR-17-92fl/fl/hUb-Cre-ERT2 mice, respectively. G, qRT-PCR analysis of the expression of the individual components of miR-17-92 gene cluster in testes of miR-17-92 KO mice (n > 5).