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. 2016 Sep;90(3):275–285. doi: 10.1124/mol.116.104570

Fig. 5.

Fig. 5.

ATP concentrations influence the effects of NPPB on WT-CFTR gating. (A) Effect of NPPB on WT-CFTR at 100 µM ATP. A continuous recording of WT-CFTR shows a decrease of macroscopic currents by lowering [ATP] from 2 mM to 100 µM. Subsequent application of NPPB results in a net decrease of the currents, indicating blocking effects outweigh the gating effect. The calculated gating effect of NPPB was 2.37 ± 0.17-fold (n = 7). (B) Similar experiments as that in panel A except [ATP] = 20 µM ATP when NPPB was added. The calculated gating effect was 3.40 ± 0.24-fold (n = 5). (C) Effect of NPPB on WT-CFTR gating at 5 µM ATP, with a calculated gating effect of 5.19 ± 0.60-fold (n = 8). (D) Effect of NPPB on Y1219F at 100 µM ATP, with a calculated gating effect of 2.95 ± 0.43-fold (n = 5). (E) Effect of NPPB on Y1219F-CFTR at 5 µM ATP, with a calculated gating effect of 9.10 ± 1.93-fold (n = 9). (F) A bar graph summarizes the gating effects of NPPB under different ATP concentrations for WT- (solid bars) or Y1219F-CFTR (open bars). Calculated gating effects of NPPB on Y1219F-CFTR at 2 mM and 20 µM ATP were 1.92 ± 0.23-fold (n = 8) and 5.41 ± 0.98-fold (n = 6), respectively.