Fig. 5.

Induction of AR degradation. C4-2 cells were pretreated with cycloheximide (20 µM) or with vehicle for 2 hours, followed by the introduction of Enz (10 µM), SAHA (10 µM), 1005 (10 µM), 2-75 (10 µM), or vehicle for the indicated durations. Cells were then harvested for Western blot analysis and probed with antibody to AR, p21, or α-tubulin (loading control). ImageJ software was used to determine the intensities of the bands relative to the 0-hour time point for each treatment. CHX, cycloheximide.