Difference in the AUC between the initial and final sympathetic responses to hypoxia-induced chemoreceptor activation. The net AUC difference in the sympathetic response to hypoxia was identified for the baseline activity and the response at 60 minutes after treatment: intermittent orexin-A or acute intermittent hypoxia (AIH). (A) The value for the net difference in AUC (final minus initial hypoxia) compared for the treatments groups involving PBS (n = 5), intermittent orexin-A 10 pmol × 10 (n = 5), and Almorexant (AXT) at 30 (n = 5) and 75 mg⋅kg−1 (n = 5). (B) Similarly, net difference in AUC between the baseline and final sympathetic response to hypoxia after AIH + PBS (n = 6), AIH + DMSO (n = 5), AIH + AXT 30 mg⋅kg−1 (n = 5), and AIH + AXT 75 mg⋅kg−1 (n = 5). For grouped data, statistical significance was determined using one-way analysis of variance followed by multiple comparisons with a Holm-Sidak correction to compare the effects with the control. All values are expressed as mean ± S.E.M. ***P < 0.005 for significance. ##P < 0.01 and ####P < 0.0001 compared with intermittent orexin-A (10 pmol × 10).