Extended Data Table 1.
Statistics of cryo-EM data collection, 3D reconstruction and model refinement.
| Data collection/processing | |
| Microscope | Krios |
| Voltage (kV) | 300 |
| Camera | Gatan K2 |
| Camera mode | Super-resolution |
| Defocus range (µm) | −1.5~−2.5 |
| Exposure time (s) | 12 |
| Dose rate (e−/pixel/s) | 8.3 |
| Magnified Pixel size (Å) | 1.35 |
| Reconstruction | |
| Software | RELION 1.4 |
| Symmetry | C2 |
| Particles refined | 26,297 |
| Resolution (unmasked, Å) | 9.0 |
| Resolution (masked, Å) | 7.3 |
| Map sharpening B-factor (Å2) | −600 |
| Model Statistics | |
| Protein residues | 2309 |
| Map CC | 0.716 |
| Resolution (FSC=0.5, Å) | 8.0 |
| MolProbity score | 2.49 |
| Cβ deviations | 0 |
| Ramachandran | |
| Outliers | 0.27% |
| Favored | 88.28% |
| RMS deviations | |
| Bond length | 0.008 |
| Bond angles | 1.26 |