Fig. 4.

Functional effects of 6-AN and 2-DG. Proliferation and migration were analyzed in the presence or absence of 6-AN (A) or 2-DG (B). GS cell proliferation was analyzed by cell counting after 7 days of incubation, and G55 proliferation was determined after 4 days using a colorimetric assay. Migration was analyzed in modified Boyden chamber assays with 24 hours (GS cells) or 5 hours (G55) of incubation. All experiments were performed at 21% O₂. Values are means ± SD of sextuplicate determinations. Asterisks indicate significant inhibition or stimulation (P < .05).