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. 2016 Mar 6;18(9):1278–1287. doi: 10.1093/neuonc/now031

Fig. 3.

Fig. 3.

In vitro efficacy of oHSV G47Δ for MN3 and other patient-derived meningioma cells. (A) Virus yield assay in MN3 cells (left, in stem cell medium) and MN298 cells (right, 10% fetal calf serum medium). Cells were infected with G207 or G47Δ at MOI = 1.25, and virus yield was measured at 24 and 48 h post-infection. Input = 10 000 pfu. *P < .0001, **P < .005. (B) Fluorescent microscopic pictures showing spread of G47Δ-mCherry (red) in MN3 cells. MN3 cells were infected at MOI 0.1 and 1, and microscopic pictures were captured at 24, 48, and 72 h p.i. Scale bars, 100 µm. (C and D) MTS cell viability assay showing the cytotoxic activity of G47Δ against MN3 cells (C) and MN7 (D). (E) MTS cell viability assay showing the cytotoxic activity of G47Δ-mCherry against different serum-cultured meningioma primary cultures in vitro. In (C–E), MTS assay was done at day 4 post oHSV infection.