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. 2016 Aug 25;11(8):e0161573. doi: 10.1371/journal.pone.0161573

Fig 2. Analysis of AvBD2 degradation products by RP-HPLC (A-E) and by antimicrobial assay (F).

Fig 2

AvBD2 was incubated in the presence of Cat B (panel A), Cat H (panel B), Cat K (panel C), Cat L (panel D), and Cat S (panel E) (S:E molar ratio = 100) in 0.1 M sodium acetate buffer, pH 5.5 containing 2 mM DTT and 0.01% Brij35 for 4 h at 30°C (black line). Control experiments used untreated AvBD2 (light grey line). Hydrolysis products were analyzed by RP-HPLC as described in Experimental Procedures. Chromatograms were recorded at 220 nm. In panel F, minimum inhibitory concentrations (MIC) in μM were calculated by radial diffusion assay for each reaction mixture towards E. coli and S. aureus, and expressed as mean ± SEM indicated in parenthesis (n = 3). NI: no inhibition detected. Significant differences of MIC values between AvBD2 and its reaction product are indicated with asterisks (*, P< 0.05).