Skip to main content
. Author manuscript; available in PMC: 2016 Aug 25.
Published in final edited form as: Nanomater Environ. 2014 Sep 1;2(1):1–12. doi: 10.2478/nanome-2014-0001

Figure 2.

Figure 2

Human embryonic stem cells maintain undifferentiated state on laminin nanofibers. A) SEM micrographs of human embryonic stem cells (huES16) cultured on laminin nanofibers (top row) on Day 1 and Day 7, and huES cultured on laminin films (bottom row) on Day 1 and Day 7. B) huES16 human embryonic stem cells grown in feeder-free culture on laminin nanofibers. SSEA-4 is a stemness marker which indicates undifferentiated cells. On laminin nanofibers, the ES cells remain about 75% undifferentiated, and take on the unique morphology shown here. C) Magnified region of (B). SSEA-4, DAPI, and overlay images shown. Scale bar in (A) = 50µm, 200µm. Scale bar in (B) = 200µm. Scale bar in (C) = 100µm.