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. 2016 Aug 25;12(8):e1005794. doi: 10.1371/journal.ppat.1005794

Fig 6. Modification of nucleotides in the fHbp 5´-UTR does not abolish thermoregulation.

Fig 6

(A) Predicted RNA secondary structure of the whole 5´-UTR and 34 bases of fHbp-mRNA. (RBS indicated in blue, start codon in red, putative α-RBSs in yellow). The nucleotides modified in pfHbp-9C-gfp are indicated. (B) Western blot analysis of fHbp-GFP levels in E. coli harbouring plasmids with modified fHbp 5´-UTRs. Bacteria were grown to mid-log phase at the indicated temperatures, and whole cell lysates subjected to Western blot analysis with antibodies recognising GFP or RecA. Relative values (rel. values) of GFP are indicated below each lane.