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. 2016 Aug 25;12(8):e1005794. doi: 10.1371/journal.ppat.1005794

Table 1. Plasmids used in this study.

Name Description/Sequence Reference
pGEM-T Vector for translational fusions, AmpR Promega
pEGFP-N2 GFP Translational fusions, KanR Clontech
pfHbp fHbp clone in pGEM-T, AmpR This study
pfHbpΔp+TSS fHbp clone in pGEM-T, without native promoter. AmpR This study
pfHbpΔp+ΔTSS-T7 fHbp clone in pGEM-T, downstream of T7 promoter, without native promoter and transcriptional start site. AmpR This study
pfHbpΔp+ΔTSS-SP6 fHbp clone in pGEM-T, downstream of SP6 promoter, without native promoter and transcriptional start site. AmpR This study
pPilE PilE clone in pGEM-T, AmpR This study
fHbp1C-GFP fHbp 5´-UTR with 1 codon clone in pEGFP-N2. KanR This study
fHbp5C-GFP fHbp 5´-UTR with 5 codon clone in pEGFP-N2. KanR This study
fHbp9C-GFP fHbp 5´-UTR with 9 codon clone in pEGFP-N2. KanR This study
fHbp9C-GFP-Mut1 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with Mut1. KanR This study
fHbp9C-GFP-Mut2 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with Mut2. KanR This study
fHbp9C-GFP-Mut3 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with Mut3. KanR This study
fHbp9C-GFP-Mut4 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with Mut4. KanR This study
fHbp9C-GFP-Mut-αRBS-1 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with MutαRBS-1. KanR This study
fHbp9C-GFP-Mut-αRBS-2 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with MutαRBS-2. KanR This study
fHbp9C-GFP-Mut-αRBS-1&2 fHbp 5´-UTR with 9 codon clone in pEGFP-N2 with MutαRBS-1&2. KanR This study
nmb1869+ fHbp9C-GFP Nmb1869+ fHbp 5´-UTR with 9 codon clone in pEGFP-N2. KanR This study
Nmb1869-KM-fHbp Nmb1869+Kan+fHBP clone in pGEM-T for mutational study in Nm. (NcoI and NotI digestion) This study