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. 2016 Aug 25;12(8):e1005794. doi: 10.1371/journal.ppat.1005794

Table 2. Table of primers.

Primer name Sequence Relevant sites
fHbp(c)-F GGTTGCCTGTAAACAAAATGC
fHbp(c)-R CGGACGGTGCAATACAAAAT
PPilE-F GGGGGAATTCCGCGCCTGTCAGATAAACC
PPilE-R GGGGCCCGGGTCGATAGGAAATCTACATCC
fHbp(TTS)-U AAATGTTTATATATTATCTATTCTGC
fHbp(SC)-U GTGAATCGAACTGCCTTCTG
fHbp-GFP-F GGGGGAATTCGGTTGCCTGTAAACAAAATGC EcoRI
fHbp-GFP1C-R GGGGCCCGGGCACAGGTTTACTCCTAGTCATACG SmaI/XmaI
fHbp-GFP5C-R GGGGCCCGGGGGCAGTTCGATTCACAGGTTTACTCC SmaI/XmaI
fHbp-GFP9C-R GGGGCCCGGGAAGGCAGCAGAAGGCAGTTCG SmaI/XmaI
nmb1869+ fHbp9C-GFP-F GGGGGAATTCAAACCTGCATACTGGCATCG EcoRI
Mut1-F CCAAAATGTTTATATACTATCTATTCTGCGTATGACTAGGAGTAAACC
Mut1-R GGTTTACTCCTAGTCATACGCAGAATAGATAGTATATAAACATTTTGG
Mut2-F CCAAAATGTTTATATATTACCTATTCTGCGTATGACTAGGAGTAAACC
Mut2-R GGTTTACTCCTAGTCATACGCAGAATAGGTAATATATAAACATTTTGG
Mut3-F CCAAAATGTTTATATTTTATCTATTCTGCGTATGACTAGGAGAAAACC
Mut3-R GGTTTTCTCCTAGTCATACGCAGAATAGATAAAATATAAACATTTTGG
Mut4-F CCAAAATGTTTATATATTATCTATTCTCCGTATGACTAGGAGTAAACC
Mut4-R GGTTTACTCCTAGTCATACGGAGAATAGATAATATATAAACATTTTGG
mutαRBS-1-F TGCGTATGACTAGGAGTAAACCTGTGAATCGAACTGGGTTCTGCTGCCTT
mutαRBS-1-R AAGGCAGCAGAACCCAGTTCGATTCACAGGTTTACTCCTAGTCATACGCA
mutαRBS-2-F TGCGTATGACTAGGAGTAAACCTGTGAATCGAACTGCCTTCTGCTGGGTT
mutαRBS-2-R AACCCAGCAGAAGGCAGTTCGATTCACAGGTTTACTCCTAGTCATACGCA
mutαRBS-2-F TGCGTATGACTAGGAGTAAACCTGTGAATCGAACTGGGTTCTGCTGGGTT
mutαRBS-2-R AACCCAGCAGAACCCAGTTCGATTCACAGGTTTACTCCTAGTCATACGCA
fHbp-U AAACGAGAAACTGAAGCTGGC
fHbp-D CACTCTCCAAGGTAATGAGC
tmRNA-U CGAAACCCAAGGTGCATGC
tmRNA-D CAGGGCTTCCACGCG
pGEM-T easy vector-F TGTAATACGACTCACTATAGGGCGAATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGCCGCGGGAT
pGEM-T easy vector-R ATTTAGGTGACACTATAGAATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCCATATGGTCGACCTGCAGGCGGCCGCACTAGTGAT
Fhbp-KM-(1)-F CCCGGCCGCCATGGCCGCGGGATGAAGAAATCGTCGAAGGCAT
Fhbp-KM-(1)-R CGATGATGGTTGGAATTCACTGGTTCAGACGGCATTTTGTTTACAGG
Fhbp-KM-(2)-F CCTGTAAACAAAATGCCGTCTGAACCAGTGAATTCCAACCATCATCG
Fhbp-KM-(2)-R AAATCAAATGTCGTCCGAACGGCGGTCGACTCTAGAGGATCCCCGGG
Fhbp-KM-(3)-F cccggggatcctctagagtcgaccGCCGTTCGGACGACATTTGATTT
Fhbp-KM-(3)-R CCTGCAGGCGGCCGCACTAGTGATCGGACTGATCCAGCGTCAAAGACTGC
PROM-10-MUT1-F CCCGGCCGCCATGGCCGCGGGATAGCTTGCGAGCCAGCGTCCGTTCC
PROM-10-MUT1-R cgtttcccgttgaatatggctcatTTGTGTCTCCTTGGGCAATAGG
PROM-10-MUT2-F CCTATTGCCCAAGGAGACACAAatgagccatattcaacgggaaacg
PROM-10-MUT2-R GCATTTTGTTTACAGGCAACCTGttagaaaaactcatcgagcatcaaatg
PROM-10-MUT3-F catttgatgctcgatgagtttttctaaCAGGTTGCCTGTAAACAAAATGC
PROM-10-MUT3-R CCTAGTCATACGCAGAATAGATAATATATAAACATTTTGGTGGTGGTATC
PROM-10-MUT4-F GATACCACCACCAAAATGTTTATATATTATCTATTCTGCGTATGACTAGG
PROM-10-MUT4-R CCTTTGTCTTTATGGTCGAGCGGTGCGGTTAGTGCATCGGCAAGCCCCGC
PROM-10-MUT5-F GCGGGGCTTGCCGATGCACTAACCGCACCGCTCGACCATAAAGACAAAGG
PROM-10-MUT5-R CCTGCAGGCGGCCGCACTAGTGAT TTATTGCTTGGCGGCAAGGCCG