Fig 7. Effects of cysteine residues of OspR on the gpx promoter activity.

Overnight cultures of ospRohrR double mutant and complementation strains carrying the gpx-lacZ reporter fusion (A) or the ohr-lacZ reporter fusion (B) were grown to mid-log phase and collected for β-galactosidase assays. Experiments were performed three times; error bars represent the standard error of the mean. White, light gray and gray bars represent control culture with no induction, sample with 500 μM cumene hydroperoxide induction and sample with 100 μM linoleic hydroperoxide induction, respectively. Significant differences (P < 0.05) between samples are denoted with asterisks.