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. 2016 Aug 25;12(8):e1006264. doi: 10.1371/journal.pgen.1006264

Fig 1. Crossing worms that contain a read-through reporter with tRNA suppressor mutants was used to report tRNA expression.

Fig 1

(A) A schematic representation of the tRNA reporter system. To monitor tRNA expression, we created worms that are homozygous for the sup mutation, the smg-2 mutation, and the reporter construct. (B) mCherry expression is a proxy for tRNA levels, and GFP reports the activity of the rps-0 promoter. Mixed-stage populations of control worms (no read-through activity) and sup-7 mutants are shown.