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. 2016 Aug 25;12(8):e1006264. doi: 10.1371/journal.pgen.1006264

Fig 2. Copies of tryptophan tRNA genes display different expression patterns.

Fig 2

(A) Representative images of young adult worms; all eight tryptophan tRNA reporter strains are displayed (90/90 worms exhibited the same expression patterns). mCherry expression is a proxy for the expression of specific tryptophan tRNA genes. Images were taken under exposure conditions that also allow detecting expression in tissues where the tRNA levels are relatively low. (B) Quantification of total worm mCherry fluorescence. All strains were analyzed when the worms were the same age (young adults), using the same exposure parameters. Shown are averages of means, ± SEM, normalized to the expression levels detected in the sup-7 strain. Three experiments were conducted, and 30 worms of each strain were scored in each experiment. *** p-value<0.001 (C) The results that were obtained using the fluorescent reporter were plotted against the Chip-seq measurements of pol III occupancy [52].