Fig 2. MdHXK1 physically interacts with MdbHLH3.

(A) The C-terminus of MdHXK1 specifically interacts with the C-terminus of MdbHLH3 in a yeast two-hybrid assay. Top panels show the schematic representation of the different MdHXK1 and MdbHLH3 deletions in yeast vectors. Bottom panels show their interaction as indicated by yeast growth and β-gal staining in a serial of yeast two-hybrid assays. (B) In vivo Co-IP assays of the interaction between MdHXK1 and MdbHLH3 in transgenic apple calli. (C) In vitro GST pull-down assays of the interaction between MdHXK1 and MdbHLH3. Anti-His immunoblot (IB) shows the amount of MdHXK1-His bound by the indicated MdbHLH3-GST protein.