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. 2016 Aug 11;16(8):2087–2101. doi: 10.1016/j.celrep.2016.07.059

Figure 2.

Figure 2

Inactivation of E-Cadherin and PTEN in Mammary Epithelium In Vivo Induces Rapid Formation of Neoplastic Lesions

(A) H&E staining and IHC of CK8 in mammary glands from 6-week-old wild-type, Wcre;Cdh1F/F, Wcre;PtenF/F, and Wcre;Cdh1F/F;PtenF/F mice. Scale bars, 150 μm.

(B) IF analysis of CK8, E-cadherin, and PTEN in mammary glands from 6-week-old Wcre;Cdh1F/F;PtenF/F mice. Scale bar, 20 μm. Zooms of the asterisk-marked area reveal CK8-positive neoplastic cells that are negative for of E-cadherin and PTEN (indicated by arrows), flanked by PTEN-positive stromal cells.

(C) IF analysis of CK8 (green) and CC3 (red) in mammary glands from 6-week-old Wcre;Cdh1F/F;PtenF/F mice. Arrows mark CC3-positive apoptotic cells in the mammary duct. Scale bar, 20 μm.

(D) Average numbers of CC3-positive shedded cells per 500-μm2 area in mammary gland sections from Wcre;Cdh1F/F, Wcre;PtenF/Fand Wcre;Cdh1F/F;PtenF/F mice.