FIGURE 1.

Expression levels of Mct8 mRNA: quantitative reverse transcriptase-mediated polymerase chain reaction (qRT-PCR) was performed with eyecup homogenates at varying age (P14, P21, P28, 6M, and 24M). Mct8 mRNA levels are given as fold-changes relative to P14 expression levels, arbitrarily set to 1. Note that normalization and statistical analysis were performed in the Ct scale (log₂), as described in “Materials and Methods.” Data are presented as fold changes, median ±25%/75% quartile and maximum/minimum value (n = 6 per age group).