. 2016 Jun 15;92(1):131–142. doi: 10.1007/s11103-016-0499-y

Table 2.

Total lignin content and phenotype in TALEN induced COMT mutant lines

Lines	Mutation frequency (%)^a	Total lignin^b (mg/g DW)	Reduction^c (%)	Stem diameter (mm)^d	Brown coloration
Lines	Mutation frequency (%)^a	Total lignin^b (mg/g DW)	Reduction^c (%)	Stem diameter (mm)^d	Internode	Midrib
WT1	0	250.2 ± 13.2	–	20.7	N	N
WT2	0	252.2 ± 4.7	–	19.9	N	N
TCA	0	251.6 ± 1.4	–	17.6*	N	N
TCB	0	257.3 ± 5.9	–	19.6	N	N
CA1	96.1	178.3* ± 28.7	30	16.3*	B	B
CA13	99.0	173.1* ± 9.8	32	15.2*	B	B
CA14	16. 8	262.9 ± 7.1	−4	17.9*	N	N
CA16	99.1	179.8* ± 10.4	29	16.3*	B	B
CA17	98.1	193.4* ± 11.2	24	16.6*	B	B
CA25	97.0	191.2* ± 9.6	24	16.1*	B	B
CB1	20.0	247.7 ± 3.4	2	20.4	N	N
CB2	7.8	240.5 ± 17.6	5	20.4	N	N
CB3	91.1	214.3* ± 12.7	15	19.6	B	N
CB5	92.2	223.0* ± 12.7	12	20	B	N
CB6	93.8	197.2* ± 14.2	22	21.4	B	B
CB7	90.0	224.1* ± 15.5	11	21.1	B	N
CB8	90.8	220.3* ± 9.4	13	19.3	B	N

WT1 and WT2 wild type sugarcane, TCA transgenic control plant generated by AIE containing only nptII gene, TCB non-mutated transgenic control plant generated by BDE harboring TALEN expression cassette, CA1–CA25 TALEN induced mutant lines generated by AIE, CB1–CB8 TALEN induced mutant lines generated by BDE, N no brown coloration observed, B brown coloration observed, DW dry weight

^aMutation frequencies were estimated by the number of sequence reads

^bValues are means ± standard deviation. Asterisk indicates a significant difference in total lignin content between WT1 and a mutant line (n = 2, P < 0.05 in t test)

^cReduction levels of lignin content in mutant lines are compared with the mean of lignin contents among control plants (WT1, WT2, TCA, and TCB)

^dValues are means of stem diameter from two different tillers of mutant lines. Asterisk indicates a significant difference in stem diameter compared with WT1 (n = 2, P < 0.05 in t test)