Fig. 3.

Western blot results obtained for different types of anti-CDK1 antibodies. The anti-CDK1 C-terminal antibody (upper panel) was applied for analysis of LSCC cell lines (left) and non-cancer head and neck tissue (right) lysates, the anti-CDK1 (phospho T161) antibody (lower panel, left) and the N-terminal anti-CDK1 antibody (lower panel, right) were applied on the LSCC cell line lysates. The numbers used are the LSCC numbers; K2, K3, K6, K12–K15 tissue sample donors numbers, L total larynx tissue lysate, J Jurkat cell line lysate, H HeLa cell line lysate. GAPDH was used as a loading control and Jurkat and HeLa cell lines were the positive controls. The samples where the trace amounts of CDK1 protein were observed are underlined