Table 1.

Oligonucleotide primers sequences and size of the PCR-targeted products.

Microorganism	Target gene	bp fragment	Primer sequence (5′–3′)	Annealing temp (°C)	Reference
E. faecalis/E. gallinarum	16S rRNA (Genus -specific primers)	112 bp	F TAC TGA CAA ACC ATT CAT GAT G	59 (E. faecalis)	Karsidani et al., 2010
			R AAC TTC GTC ACC AAC GCG AAC	50 (E. gallinarum)
	vanA (Glycopeptide resistance genotype Vancomycin resistance)	732 bp	F GGG AAA ACG ACA ATT GC	59 (E. faecalis)	Biavasco et al., 2007; Karsidani et al., 2010
			R GTA CAA TGCG GCC GTTA	50 (E. gallinarum)
	vanC1 (Glycopeptide resistance genotype Vancomycin resistance)	822 bp	F GGT ATC AAG GAA ACC TC	59 (E. faecalis)
			R CTT CCG CCA TCA TAG CT	50 (E. gallinarum)
E. faecalis	ddlE.faecalis (Species-specific primers)	941 bp	F ATC AAG TAC AGT TAG TCT	55
			R ACG ATT CAA AGC TAA CTG
	esp (virulence factor)	932 bp	F TTG CTA ATG CTA GTC CAC GACC	55
			R GCG TCA ACA CTT GCA TTG CCG AA