Table 1.
Microorganism | Target gene | bp fragment | Primer sequence (5′–3′) | Annealing temp (°C) | Reference |
---|---|---|---|---|---|
E. faecalis/E. gallinarum | 16S rRNA (Genus -specific primers) | 112 bp | F TAC TGA CAA ACC ATT CAT GAT G | 59 (E. faecalis) | Karsidani et al., 2010 |
R AAC TTC GTC ACC AAC GCG AAC | 50 (E. gallinarum) | ||||
vanA (Glycopeptide resistance genotype Vancomycin resistance) | 732 bp | F GGG AAA ACG ACA ATT GC | 59 (E. faecalis) | Biavasco et al., 2007; Karsidani et al., 2010 | |
R GTA CAA TGCG GCC GTTA | 50 (E. gallinarum) | ||||
vanC1 (Glycopeptide resistance genotype Vancomycin resistance) | 822 bp | F GGT ATC AAG GAA ACC TC | 59 (E. faecalis) | ||
R CTT CCG CCA TCA TAG CT | 50 (E. gallinarum) | ||||
E. faecalis |
ddlE.faecalis (Species-specific primers) |
941 bp | F ATC AAG TAC AGT TAG TCT | 55 | |
R ACG ATT CAA AGC TAA CTG | |||||
esp (virulence factor) |
932 bp | F TTG CTA ATG CTA GTC CAC GACC | 55 | ||
R GCG TCA ACA CTT GCA TTG CCG AA |