Table 1. ICD activity in cell-free extracts of bacteria grown on glucose or acetate.

Bacterial strain	ICD activity* Glucose (Glc)	ICD activity* Acetate (Ace)	Ratio (Glc/Ace)
M. bovis BCG	0.65±0.09	1.01±0.14	0.64
BCG Δicd1	0.63±0.08	1.12±0.13	0.56
BCG Δicd2	ND†	ND†
BCG Δicd2 attB::Pnpicd2‡	1.55±0.06	2.91±0.83	0.53
M. smegmatis	0.84±0.24	2.08±0.13	0.40
M. smegmatis Δicd2	0.01±0.01	0.02±0.01	0.50
M. smegmatis Δicd attB::Pnpicd†	1.02±0.18	3.19±0.42	0.32

Data are means±s.d. (n=3 independent experiments each performed in triplicate).

^*ICD specific activity in cell-free extracts (U μg⁻¹).

^†Not detectable.

^‡Strain complemented with single-copy pMV306-based plasmids integrated at the chromosomal attB site. In each case, the complementing gene is expressed from the native promoter (P_np).