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. 2016 Aug 23;7(4):e01055-16. doi: 10.1128/mBio.01055-16

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Copyright © 2016 Roelofs et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Identification of BSAP-2. (A) ORF map of a B. uniformis CL03T00C23 (BuCL03) region showing a transposon (tn) insertion resulting in loss in antimicrobial activity against B. uniformis 8492 (Bu8492). (B to F) Agar overlay assays showing growth or inhibition of Bu8492 by (B) BuCL03 wild type (wt) and transposon mutant tn::1165. (C) The BUCL03 wt and deletion mutants of HMPREF1072_1165-7 (Δ1165-7) or HMPREF1072_1167 (Δ1167), (D) BuCL03 Δ1165-7 and Δ1167 mutants containing empty vector (vector) or plasmid-expressing HMPREF1072_01167 (1167), (E) B. fragilis (B.f.) CM11 with empty vector or 1167, (F) and a phosphate-buffered saline (PBS) control or purified His-tagged 1167 (His-1167).