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Saccharification of microcrystalline cellulose (MCC) with MCC-adapted culture supernatants at 60°C after 72 h. Supernatants were collected from each culture and incubated with 2% (wt/vol) Avicel. Enzyme loading was set at 5 mg enzyme/g glucan as measured by the BCA assay. Glucose and cellobiose were measured by HPLC. Error bars represent standard deviations from the mean from three technical replicates.
