FIG 4 .

Production of cellobiose from Avicel by T. bispora cellulases expressed in E. coli. Mix3 (GH12, GH6_exo, and GH48) and Mix4 (GH12, GH6_exo, GH48, and GH6_endo) represent samples mixed at equal concentrations of each protein. Mix3 Add and Mix4 Add represent sums of individual activities of each protein at the specified concentration. Proteins were purified and saccharifications were performed as described in Materials and Methods. Glucose and cellobiose were measured by HPLC, although only cellobiose is reported in the figure. Measured glucose release is depicted in Fig. S2 in the supplemental material.