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qRT-PCR analysis confirms PcVelA-dependent expression of putative MTase-encoding genes. Analysis of relative log2-fold gene expression ratios of putative MTase-encoding genes confirmed PcVelA dependency. Ratios of their expression in PcVelA-ChIP (gray bars) and the ΔPcvelA strain (black bars) are shown compared to that in wild-type P2niaD18. Values are the mean scores from three biological replicates. Partial amplification of the 18S rRNA was used as a reference for normalization.
