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. 2016 Aug 26;6:32283. doi: 10.1038/srep32283

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The BmGP64 gene was disrupted by a cat cassette using the Red recombination system to construct the ΔBmNPV bacmid. BmNPVΔbgp/AcGP64/EGFP and BmNPVΔbgp/BmGP64/EGFP bacmids were constructed using the Bac-to-Bac system and pFastbacdual/p10-AcGP64/CMV-EGFP and pFastbacdual/p10-BmGP64/CMV-EGFP, respectively, as shown.