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. 2016 Aug 26;6:32283. doi: 10.1038/srep32283

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Each virus was propagated on Bm5 (BmNPVΔbgp/AcGP64/EGFP and BmNPVΔbgp/BmGP64-EGFP) or Sf-9 (BacMam 2.0) cells and partially purified. Subsequently, 1 × 10⁸ or 1 × 10⁷ PFU of each virus was separated by SDS-PAGE, transferred to a PVDF membrane, and subjected to western blot analysis using rabbit anti-BmNPV GP64 polyclonal antibody. Lane 1: BmNPVΔbgp/AcGP64/EGFP, Lane 2: BmNPVΔbgp/BmGP64-EGFP, Lane 3: BacMam 2.0. Arrows indicate expressed AcGP64 or BmGP64.