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. 2016 Aug 26;6:32108. doi: 10.1038/srep32108

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Observation hives used for 1-frame and 2-frame experimental hives containing 1500 and 3000 worker bees and a laying queen, respectively (A). Ulster (U501) observation hive for 5-frame experimental hives containing roughly 7000 worker bees and a laying queen. The frame containing the laying queen was placed in the upper level during observation periods while the other four frames and a feeder remained in the lower portion of the box. The entrance of the hive is located in the lower level and is shown taped closed in this picture (B).