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. 2016 Aug 26;6:32128. doi: 10.1038/srep32128

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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NIH3T3 cells were cultured in 6-well plates and infected with the indicated viruses at an MOI of 1, or were left uninfected. For each condition, three biological replicates were done. Total RNA was isolated 48 hr p.i., and the analysis of the expression of the MHV-68 miRNAs was performed by LC Sciences (Houston, Texas, USA) using custom miRNA microarrays. The heatmap was generated using the matrix2png web interface⁶⁴.