Skip to main content
. 2016 Aug 26;6:32111. doi: 10.1038/srep32111

Figure 2. AICAR inhibits ER stress responses.

Figure 2

(A) mRNA expression of ER stress markers, (B,C) Western analysis of cell lysates (B) or nuclear extracts (C).(D) mRNA expression of ATF4 and ATF6α in macrophages pre-exposed for 1 h to AICAR and treated with thapsigargin or tunicamycin for 6 h. (E) Western analysis of macrophages pre-exposed for 1 h to 0.5 mM AICAR in the absence or presence of ABT-702 and treated with thapsigargin for 6 h. (F) mRNA expression of CHOP and Grp78 in macrophages pre-exposed for 1 h to indicated concentrations of AICAR in the absence or presence of ABT-702 and treated with thapsigargin or tunicamycin for 6 h. (G) Protein expression of AMPK 72 h post-transfection with control siRNA or AMPKα1 siRNA. (H) mRNA expression of CHOP, Grp78 and AMPKα1 in macrophages transfected with control siRNA or AMPKα1 siRNA for 72 h prior to treatments with thapsigargin for 6 h with or without 1 h AICAR pre-exposure. *p < 0.05. Data represent mean values ± SE of at least three independent experiments. ns, non-specific band. Western Blot images are cropped scans either of the same or of the duplicate membranes probed with different antibodies.