Figure 3.

Schematic of replication fork regression and its regulation in yeast. Stalled replication forks can be reversed through the action of DNA helicases, such as Rad5, Mph1, and others, as described in the text. The regressed fork can be processed further, for example undergoing end resection or DNA synthesis. One possible outcome entails the invasion of homologous template strands, leading to the generation of recombination intermediates in the forms of DNA joint molecules, which will need to be processed before chromosome segregation. The role of Mph1 in fork regression is inhibited by the Smc5/6 complex, and MHF and associated Mte1 help alleviate this effect.