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. 2016 Aug 17;7(8):49. doi: 10.3390/genes7080049

Figure 3.

Figure 3

Epigenetic changes induced by 5-azadeoxycytidine (5-azadC) treatment. The use of 5-azadC on different FXS lymphoblasts produce FMR1 transcript reactivation as shown by RT-PCR, and a partial rescue of translation demonstrated by immunocytochemistry (Panel A). Reactivation was quantified by real time PCR and the percentage of demethylated clones was evaluated by bisulphite sequencing of the CpG island of the FMR1 promoter region (Panel B). The effects of 5-azadC on histone marks (acetylation of H3 and H4, increased methylation of H3K4) are reported in panel C. Data derived from Refs. [42,65,66].