Figure 1. Regulation of PHT folate uptake byTORC1 and 2.

(a) Time course of ³H-5-MTHF uptake in the absence and presence of 1.5 mM unlabeled MTHF in cultured human primary trophoblast (PHT) cells. Uptakes were linear for at least 120 min. Values are given as means ± SEM. n = 4. (b) Inhibition of mTOR by 100 nM rapamycin decreased the basal folate uptake in PHT cells. (c) Effect of silencing raptor (mTORC1 inhibition) or rictor (mTORC2 inhibition) on folate uptake. (d) Effect of simultaneous silencing of raptor and rictor on basal folate uptake. Uptake of [3′, 5′, 7, 9-³H]-Methyltetrahydrofolic acid was measured for 80 minutes at 37 °C. Values are means + SEM; n = 3–6. *P < 0.05 versus control; paired Student’s t test (b,d) RMANOVA with Tukey–Kramer multiple comparisons post hoc test; means without a common letter differ significantly (P < 0.05).