Figure 3. Growth factors stimulates folate uptake in PHT cells mediated by mTORC2.

(a) The stimulatory effect of growth factors [Insulin (5.8 ng ml⁻¹) + IGF-1 (300 ng ml⁻¹)] on ³H-5-MTHF uptake was not significantly influenced by raptor silencing. (b) In contrast, rictor silencing in PHT cells prevented growth factor-stimulated folate uptake. Values are means + SEM; n = 5. Means without a common letter differ significantly (P < 0.05) by RMANOVA with Tukey–Kramer multiple comparisons post hoc test.