Figure 6. Effect of scopolamine butylbromide on histological and morphometric analysis of the pancreas.

Representative images (a,d) × 20 magnification, scale bars = 1000 μm; (e,h) × 400 magnification, scale bars = 50 μm) of pancreatic sections immunostained with an anti-insulin antibody. NL-S, normal litter rats treated with saline solution (a,e); SL-S, small litter rats treated with saline solution (b,f); NL-B, normal litter rats treated with scopolamine butylbromide (c,g); SL-B, small litter rats treated with scopolamine butylbromide (d,h). A quantitative analysis of islet number, area and insulin immunodensity is shown in (i–k), respectively. Data are presented as the mean ± SEM, obtained from 4 rats in each experimental group. A total of 160 islets were analyzed per group. L, litter size factor; T, treatment factor; and I, interaction between litter size and treatment factors. *P < 0.05, **P < 0.01 and ***P < 0.001, based on a two-way analysis of variance. ^ɸP < 0.05 and ^ɸɸP < 0.01, statistical significance of the differences between NL and SL; ^#P < 0.05, statistical significance of saline versus scopolamine butylbromide animals, based on the Tukey multiple comparisons test.