Figure 2.

Selected marine drug activated p53 family members in human tumor cells. Tumor cells (SCC-11, M87-MG, and RKO) were treated with control media (−) and chromomycin A2 (+, 30 nM, panel A), psammaplin A (+, 7.5 µM, panel B) and ilimaquinone (+, 10 µM, panel C) for 16 h. Total protein lysates were analyzed by immunoblotting with indicated antibodies against ΔNp63α TAp73α and wild type (wt)-TP53, as well as against p(S385)-ΔNp63α, p(Y99)-TP73, p(S15)-TP53, and p(S46)-TP53. Level of β-tubulin served as a loading control.