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. 2016 Aug 16;14(8):154. doi: 10.3390/md14080154

Figure 4.

Figure 4

Expression of autophagic genes (ATG7, ATG10, ATG5, UVRAG, BECN1, and ULK1) in tumor cells treated with marine drugs. (A) Expression of indicated autophagic genes (ATG7, ATG10, ATG5, UVRAG, BECN1, and ULK1) in tumor cells upon exposure to marine drugs. QPCR analysis of gene targets in SCC-11 cells, U87-MG cells, and RKO cells. Three independent qPCR assays were performed in triplicate (± SD are indicated. * p < 0.05); (B) Luciferase reporter assay of target gene promoters (ATG7, ATG10, ATG5, UVRAG, BECN1, and ULK1). Tested cells were transfected for 24 h with 100 ng of the LightSwitch_Pro reporter plasmids containing the indicated autophagic promoters or with 100 ng of the control promoter-less reporter plasmid. Cells were exposed to control medium (Con) or medium with chromomycin A2 (CA2, 30 nM), psammaplin A (PMA, 7.5 µM), and ilimaquinone (ILQ, 10 µM) for 12 h. RenSP Renilla luciferase reporter activity assays were conducted using three independent biological experiments in triplicate (± SD are indicated. * p < 0.05). Data is presented as relative to the data obtained from the control untreated cells containing the promoter-less reporter plasmid designated as 1.