Figure 5.

TP53 family members bound to the tested autophagic gene promoters upon exposure to the indicated marine drugs. Tumor cells were exposed to control medium (Con) or medium with chromomycin A2 (CA2, 30 nM), psammaplin A (PMA, 7.5 µM), and ilimaquinone (ILQ, 10 µM) for 12 h. Chromatin imunoprecipitation (ChIP) of the TP53, TP63, and TP73 (using indicated antibodies) bound to the specific regions (A) and nonspecific regions (B) of the indicated autophagic gene promoter sequences. Quantitative real-time qPCR experiments were performed in triplicate with ±SD as indicated (* p < 0.05). The amount of immunoprecipitated-enriched DNA in each sample (ChIP) is represented as a signal relative to the total amount of input chromatin DNA (Input) using the same primers multiplied by 100. Assays were performed using three independent biological experiments in triplicate.