FIG 7 .

Amelioration of killing by 5-fluorouridine is specific to pyoverdine-mediated pathology. (A) Dose-response curve of C. elegans killing mediated by the P. aeruginosa PA14 pvdA mutant (which is defective in pyoverdine biosynthesis) after exposure to various concentrations of 5-fluorouracil (FU), 5-fluorouridine (FUR), or 5-fluorocytosine (FC). (B) C. elegans survival during a lethal, plate-based, P. aeruginosa infection assay. Plates were supplemented with FU, FUR, or FC at the concentrations shown. Pyoverdine was dispensable for killing in this assay. *, P < 0.01; #, P < 0.05 (based on Student’s t test) (A); P < 0.05 (for 4 µM FU, based on log-rank test [http://bioinf.wehi.edu.au/software/russell/logrank/]) (B). Three (A) or two (B) biological replicates were done for each experiment. Data from representative replicates are shown. d, day.