FIG 4.

Each component of the micrococcin biosynthetic pathway is amenable to affinity tagging. (A) Graphical representation of the affinity tags fused to the N terminus of the precursor peptide TclE. Spot-on-lawn assays were used to test the bioactivity resulting from tagged TclE. (B) Representations of the various locations in which Strep tags (ST) were fused to Tcl-processing proteins. The effect of these tags on bioactivity is also indicated; +, the fusion protein was functional; −, the fusion abolished bioactivity; NT, the tag was not tested.