TABLE 3.

Comparison of pilin antigenic variation frequencies

Strain, mutant genotype, or conditiona	Relative pilin Avb determined by:		% Av determined from 454 sequencing
	Previous methodsc	454 sequencing
Parent 1	+++	+++	10.58
Parent 2	+++	+++	10.81
pilC1PL	+++	+++	12.77
recA9d	−	−	−
recO	−	−	−
recQ	+	+	2.61
rep	++	++	5.46
recN	+++	+++	12.43
recB	+++	++++	19.00
recJ	+	+	3.19
recX	+	+	3.44
rdgC	++	++	8.42
recG	+	++	7.74
ruvB	+	+++	12.48
recAECd	++	++	6.21
recAXECd	++++	++++	17.04
Avd-1	−	−	−
Avd92	−	−	−
Avd-5-4-1	+	+	1.73
Av-A17T	ND	+++	11.67
−35 mut	++	+	4.44
Desferal	++++	++++	14.40
thrB	++	++	7.47

^aAll strains are in the FA1090 recA6 background, except as noted.

^b−, Av deficient; +, severely deficient; ++, moderately deficient; +++, parental levels of variation; ++++, increased levels of Av; ND, not determined.

^cPreviously analyzed by PDCMC assay, PCR, RT-PCR, percent nonpiliated, and direct sequencing (18, 21, 22, 24–26, 34–36, 38, 41, 43, 46, 56, 61, 62, 65).

^dFA1090 recA⁺ background.