FIG 1.

Effects of dgcZ and cpxR deletions on attachment of E. coli MG1655. (A) Western blot detection of DgcZ in E. coli MG1655 dgcZ(wt) and ΔdgcZ strains grown for 24 h at 30°C under static conditions. (B) Attachment assay of MG1655, ΔdgcZ, ΔcpxR, and ΔdgcZ ΔcpxR strains grown as described for panel A. The results are averages for 12 wells inoculated with single colonies in two different experiments. Error bars indicate the standard errors of the mean (SEM). Statistic analysis was performed using a Student t test for two independent means, where each P value was obtained by comparing the mutant to the MG1655 strain.