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. 2016 Aug 26;6:32405. doi: 10.1038/srep32405

Figure 1. Aim2 is expressed in neurons and is involved in IL-1β secretion.

Figure 1

(a–g) Expression of a series of inflammasome-related genes in mouse cortical and hippocampal mixed cultures (CN + HN) at 4 days in vitro (DIV) and in mouse brains, examined by quantitative-PCR using both the Universal ProbeLibrary (a,c,e,f,g) and the SYBR Green (b,d) systems. The ages of examined mice are indicated. In cultured neurons, Cyp was used as internal control. In brains, Gapdh was used as internal control because its expression levels were more constant in brains of different ages. (e) Absolute copy number of Aim2, Nlrp3 and Nlrc4 transcripts in adult brains. (h) Activation of TLR7 and AIM2 induces IL-1β secretion. Cortical and hippocampal mixed cultures were pretreated with CL075 (6 μM) to activate TLR7 for 6 h and then transfected with poly dAdT (0, 2 or 5 μg) for a further 4 h. The levels of secreted IL-1β in cultured media were measured using ELISA. (h) left, WT; right, Aim2−/−. The data are presented as the mean ± s.d. *p < 0.05; **p < 0.01; ***p < 0.001. The experiments were repeated independently three times.