FIGURE 2.
Nucleotide dependent dimerization of AcsFCh. A, the elution profile of monomeric AcsFCh without nucleotide is shown as solid line. Monomeric AcsFCh was incubated with either MgADP or MgATP and the oligomeric state was analyzed by SEC. The elution profiles of AcsFCh in the presence of MgADP or MgATP are shown as dashed and dotted lines, respectively. B, influence of nucleotide on the oligomeric state of K10A-AcsFCh. The elution profile of monomeric K10A-AcsFCh is shown as solid line and the elution profile of K10A-AcsFCh in the presence of MgADP or MgATP are shown as dashed and dotted lines, respectively. All dimerization experiments were at least performed with two independent samples.