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. 2016 Jul 8;291(35):18139–18147. doi: 10.1074/jbc.M116.737734

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — A, splenocyte/IFNγ release assay comparing the biological activity of mouse IL12-derived split cytokine derivatives alone or in combination 48 h after sample addition. B, comparative pSTAT4 activation assay revealing the signaling capacity of IL12-derived split cytokine derivatives 25 min after sample addition. The sample mixtures were premixed in a molar 1-to-1 ratio 30 min before the start of the respective assay. Mean fluorescence intensities (MFI) are displayed. Experiments were analyzed in triplicates. Error bars indicate ± S.D.