FIGURE 1.

Down-regulation of KLHL21 in macrophages treated with proinflammatory stimuli. The mRNA level of Klhl21 in RAW264.7 cells (A) and in bone marrow-derived macrophages (B) treated with 100 ng/ml LPS at different time points were determined by quantitative RT-PCR, respectively (n = 3). C, the protein level of KLHL21 in RAW264.7 cells stimulated with LPS was analyzed by Western blotting (n = 3). D–F, RAW264.7 cells were treated with 100 ng/ml Pam2CSK4 and Pam3CSK4 (D), 5 μm ODN1826 (E), and 20 ng/ml TNFα (F), respectively. The mRNA and protein level of KLHL21 were analyzed by using real-time PCR (n = 3) and Western blotting, respectively. IB, immunoblotting. Error bars, S.E.