Skip to main content
. 2016 Jul 1;291(35):18199–18209. doi: 10.1074/jbc.M116.717793

FIGURE 4.

FIGURE 4.

HDAC6 is required for Btz-induced aggresome formation in HNSCC cells. A, real time RT-PCR showing the mRNA level of HDAC6 in SCC1 cells infected with lentiviruses expressing HDAC6 shRNA vector or scramble. **, p < 0.01. B, microscopic images of aggresome using anti-ubiquitin and anti-vimentin antibodies and DAPI staining in HDAC6 knockdown SCC1 cells and control cells treated with Btz for 24 h. Scramble, SCC1 cells infected with lentiviruses expressing scramble shRNA; HDAC6KD, SCC1 cells infected with lentiviruses expressing HDAC6 shRNA. Scale bar, 15 μm. C, bar graph represents the average number of aggresome per 100 cells in HDAC6KD SCC1 cells and control cells treated with Btz for 24 h. Values are means ± S.D.; **, p < 0.01. Data were collected from three independent experiments. D, real time RT-PCR showing the mRNA level of HDAC6 in SCC23 cells infected with lentiviruses expressing HDAC6 shRNA vector or scramble. **, p < 0.01. E, microscopic images of aggresome using anti-ubiquitin and anti-vimentin antibodies and DAPI staining in HDAC6KD SCC23 cells and control cells treated with Btz for 24 h. Scale bar, 15 μm. F, bar graph represents the average number of aggresome per 100 cells in HDAC6KD SCC23 cells and control cells treated with Btz for 24 h. Values are means ± S.D.; ** p < 0.01, Data were collected from three independent experiments.